Composite

Part:BBa_K3408011:Design

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-09)


PliaG-B0034-lacI-Pgrac-B0034-CⅠ-PCⅠ-GFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2155


Design Notes

We used this part to verify the feasibility of our IPTG induction system in our engineered Bacillus subtilis.

Source

We designed it by combining our basic part optimized CⅠ(BBa_K3408004).

References