Part:BBa_K3408011:Design

P_liaG-B0034-lacI-P_grac-B0034-CⅠ-P_CⅠ-GFP-B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1294
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2155
  

Design Notes

We used this part to verify the feasibility of our IPTG induction system in our engineered Bacillus subtilis.

Source

We designed it by combining our basic part optimized CⅠ(BBa_K3408004).